Publications

2017

*Thomas, L.L., *Joiner, A.M.N., and Fromme, J.C. The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway. J. Cell Biol. 2017 Nov. 6 (epub)  *Denotes co-first authors

Gustafson, M.A., and Fromme, J.C. Regulation of Arf activation occurs via distinct mechanisms at early and late Golgi compartments. Mol. Biol. Cell 2017 Oct. 4 (epub)

2016

Thomas, L.L., and Fromme, J.C. GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis. J. Cell Biol. 2016 Nov 21;215(4):499-513. doi: 10.1083/jcb.201608123.

Paczkowski, J.E. and Fromme, J.C. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor. Methods Mol Biol. 2016;1496:41-53. doi: 10.1007/978-1-4939-6463-5_4. 

Richardson, B.C., Halaby, S.L., Gustafson, M.A., and Fromme, J.C. The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase. eLife.10.7554/eLife.12411.

2015

Tang, S., Henne, W.M., Borbat, P.J., Buchkovich, N.J., Freed, J.H., Mao, Y., Fromme, J.C., Emr, S.D. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. eLife. 10.7554.

Richardson, B.C and Fromme, J.C. Biochemical methods for studying kinetic regulation of Arf1 activation by Sec7. Methods Cell Biol. 130:101-26. doi: 10.1016/bs.mcb.2015.03.020. Epub 2015 Jun 11.

Paczkowski, J.E., Richardson, B.C., and Fromme, J.C.  Cargo adaptors: structures illuminate mechanisms regulating vesicle biogenesis. Trends Cell Biol. 10.1016/j.tcb.2015.02.005

Fromme, J.C.  Membrane trafficking: licensing a cargo receptor for ER export. Curr. Biol. 25(2): R67-8.

2014
McDonold, C.M., and Fromme, J.C.  Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-Golgi network. Dev. Cell. 30: 759-767.

Paczkowski, J.E., and Fromme, J.C.  Structural basis for membrane binding and remodeling by the exomer secretory vesicle cargo adaptor. Dev. Cell. 30: 610-624.

Weiskoff, A.M., and Fromme, J.C.  Distinct N-terminal regions of the exomer secretory vesicle cargo Chs3 regulate its trafficking itinerary. Front. Cell Dev. Biol. 2: 10.3389/fcell.2014.00047.

Fromme, J.C. and Segev N.  Regulation of vesicle trafficking: GTPases and friends. Mol. Biol. Cell. 25(6):732


2013
Richardson, B.C., and Fromme, J.C.  The exomer cargo adaptor features a flexible hinge domain. Structure 21(3):486-92.


2012
*Paczkowski, J.E., *Richardson, B.C., Strassner, A.M., and Fromme, J.C.  The exomer cargo adaptor structure reveals a novel GTPase-binding domain. EMBO J. 31:4191-4203. *Denotes co-first authors

Richardson, B.C., and Fromme, J.C.,  Autoregulation of Sec7 Arf-GEF activity and localization by positive feedback. Small GTPases. 3: 240-243.

Richardson, B.C., McDonold, C.M., Fromme, J.C.  The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback.Dev. Cell. 22:799-810.

Fromme, J.C., Kim, J.  A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters. Anal. Biochem. 421:482-488.

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