2022
Bagde, S.R., Fromme, J.C. Structure of a TRAPPII-Rab11 activation intermediate reveals GTPase substrate selection mechanisms. Sci Adv. doi: 10.1126/sciadv.abn7446.
2021
Sardana, R., Highland, C.M., Straight, B.E., Chavez, C.F., Fromme, J.C., Emr, S.D. Golgi membrane protein Erd1 essential for recycling a subset of Golgi glycosyltransferases. Elife. doi: 10.7554/eLife.70774.
Humphreys, I.R., Pei, J., Baek, M., Krishnakumar, A., Anishchenko, I., Ovchinnikov, S., Zhang, J., Ness, T.J., Banjade, S., Bagde, S.R., Stancheva, V.G., Li, X.H., Liu, K., Zheng, Z., Barrero, D.J., Roy, U., Kuper, J., Fernandez, I.S., Szakal, B., Branzei, D., Rizo, J., Kisker, C., Greene, E.C., Biggins, S., Keeney, S., Miller, E.A., Fromme, J.C., Hendrickson, T.L., Cong, Q., Baker, D. Computed structures of core eukaryotic protein complexes. Science. doi: 10.1126/science.abm4805.
Bagde, S.R., Mathews, I.I., Fromme, J.C., Kim, C-Y. Modular polyketide synthase contains two reaction chambers that operate asynchronously. Science. doi: 10.1126/science.abi8532.
Lormand, J.D., Kim, S-K., Walters-Marrah, G.A., Brownfield, B.A., Fromme, J.C., Winkler, W.C., Goodson, J.R., Lee, V.T., Sondermann, H. Structural characterization of NrnC identifies unifying features of dinucleotidases. Elife. doi: 10.7554/eLife.70146.
Nguyen, T., Richards, A.F., Neupane, D.P., Feathers, J.R., Yang, Y., Siim, J.H., Byuan, H., Lee, S., Ahn, C., Van Slyke, G., Fromme, J.C., Mantis, N.J., Song, J. The structural basis of Salmonella A2B5 toxin neutralization by antibodies targeting the glycan-receptor binding subunits. Cell Rep. doi: 10.1016/j.celrep.2021.109654.
Joiner, A.M.N., Phillips, B.P., Yugandhar, K., Sanford, E.J., Smolka, M.B., Yu, H., Miller, E.A., Fromme, J.C. Structural basis of TRAPPIII-mediated Rab1 activation. The EMBO Journal. E107607.
Highland, C.M., Fromme, J.C. Arf1 directly recruits the Pik1-Frq1 PI4K complex to regulate the final stages of Golgi maturation. Mol Biol Cell. 32(10): 1064-1080.
Thomas, L.L., Highland, C.M., Fromme, J.C. Arf1 orchestrates Rab conversion at the trans-Golgi network. Mol. Biol. Cell. 32(11):1081-1133.
Joiner, A.M.N., Fromme, J.C. Structural basis for the initiation of COPII vesicle biogenesis. Structure. doi: 10.1016/j.str.2021.03/013.
Feathers, J.R., Spoth, K.A., Fromme, J.C. Experimental evaluation of super-resolution imaging and magnification choice in single-particle cryo-EM. J. Struct. Biol. X. 5:100047
Lanz, M.C., Yugandhar, K., Gupta, S., Sanford, E.J., Faca, V.M., Vega, S., Joiner, A.M.N., Fromme, J.C., Yu, H., Smolka, M.B. In-depth and 3-dimensional exploration of the budding yeast phosphoproteome. EMBO Rep. 22(2):e51121.
2020
Thomas, L.L., Fromme, J.C. Extensive GTPase crosstalk regulates Golgi trafficking and maturation. Curr Opin Cell Biol. 65:1-7.
Kosciuk, T., Price, I.R., Zhang, X., Zhu, C., Johnson, K.N., Zhang, S., Halaby, S.L., Komaniecki, G.P., Yang, M., DeHart, C.J., Thomas, P.M., Kelleher, N.L., Fromme, J.C., Lin, H. NMT1 and NMT2 are lysine myristoyltransferases regulating the ARF6 GTPase cycle. Nat Commun. 11(1):1067.
2019
Thomas, L.L., van der Vegt, S.A., Fromme, J.C. A Steric Gating Mechanism Dictates the Substrate Specificity of Rab-GEF. Dev Cell. 48(1):100-114.e9.
2018
Halaby, S.L. and Fromme, J.C. The HUS-box is required for allosteric regulation of the Sec7 Arf-GEF. J. Biol. Chem. 293(18):6682-6691.
*Thomas, L.L., *Joiner, A.M.N., and Fromme, J.C. The TRAPPIII complex activates the GTPase Ypt1 (Rab1) in the secretory pathway. J. Cell Biol. 217(1):283-298. *Denotes co-first authors
2017
Gustafson, M.A., and Fromme, J.C. Regulation of Arf activation occurs via distinct mechanisms at early and late Golgi compartments. Mol. Biol. Cell 28(25):3660-3671.
2016
Thomas, L.L., and Fromme, J.C. GTPase cross talk regulates TRAPPII activation of Rab11 homologues during vesicle biogenesis. J. Cell Biol. 215(4):499-513.
Paczkowski, J.E. and Fromme, J.C. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor. Methods Mol Biol. 2016;1496:41-53.
Richardson, B.C., Halaby, S.L., Gustafson, M.A., and Fromme, J.C. The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase. eLife.10.7554/eLife.12411.
2015
Tang, S., Henne, W.M., Borbat, P.J., Buchkovich, N.J., Freed, J.H., Mao, Y., Fromme, J.C., Emr, S.D. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. eLife. 10.7554.
Richardson, B.C and Fromme, J.C. Biochemical methods for studying kinetic regulation of Arf1 activation by Sec7. Methods Cell Biol. 130:101-26. doi: 10.1016/bs.mcb.2015.03.020. Epub 2015 Jun 11.
Paczkowski, J.E., Richardson, B.C., and Fromme, J.C. Cargo adaptors: structures illuminate mechanisms regulating vesicle biogenesis. Trends Cell Biol. 10.1016/j.tcb.2015.02.005
Fromme, J.C. Membrane trafficking: licensing a cargo receptor for ER export. Curr. Biol. 25(2): R67-8.
2014
McDonold, C.M., and Fromme, J.C. Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-Golgi network. Dev. Cell. 30: 759-767.
Paczkowski, J.E., and Fromme, J.C. Structural basis for membrane binding and remodeling by the exomer secretory vesicle cargo adaptor. Dev. Cell. 30: 610-624.
Weiskoff, A.M., and Fromme, J.C. Distinct N-terminal regions of the exomer secretory vesicle cargo Chs3 regulate its trafficking itinerary. Front. Cell Dev. Biol. 2: 10.3389/fcell.2014.00047.
Fromme, J.C. and Segev N. Regulation of vesicle trafficking: GTPases and friends. Mol. Biol. Cell. 25(6):732
2013
Richardson, B.C., and Fromme, J.C. The exomer cargo adaptor features a flexible hinge domain. Structure 21(3):486-92.
2012
*Paczkowski, J.E., *Richardson, B.C., Strassner, A.M., and Fromme, J.C. The exomer cargo adaptor structure reveals a novel GTPase-binding domain. EMBO J. 31:4191-4203. *Denotes co-first authors
Richardson, B.C., and Fromme, J.C., Autoregulation of Sec7 Arf-GEF activity and localization by positive feedback. Small GTPases. 3: 240-243.
Richardson, B.C., McDonold, C.M., Fromme, J.C. The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback.Dev. Cell. 22:799-810.
Fromme, J.C., Kim, J. A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters. Anal. Biochem. 421:482-488.